Template:SJTU-BioX-Shanghai/Notebook/Transformation/w9

Transformation

pdark natural transformation

In the evening of Aug 31

3rd liquid segregation of pdark-HR

In the evening of Sep 3

4th liquid segregation of pdark-HR

In the evening of Sep 6

5th liquid segregation of pdark-HR

pcpcG2 electro-transformation

in the evening of 2nd, Sep

2nd liquid segregation of pcpcG2-HR

in the evening of 4th, Sep

3rd liquid segregation of pcpcG2-HR

in the evening of 6th, Sep

4th liquid segregation of pcpcG2-HR

second desalination test of pdark-HR

on 2nd, Sep

inoculated 24 wildtype & 24 pdark-HR (use pdark-HR from colony 3) at 9pm

on 4th, Sep

induced with darkness at 9pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each)

on 5th, Sep

cultivated with white light at 9am, then took the second 6 samples,

took the third 6 samples at 10am

took the fourth 6 samples at 11am

took the fifth 6 samples at 1pm

took the sixth 6 samples at 9pm

on 6th, Sep

took the seventh 6 samples at 9am

took the eighth 6 samples at 9pm