Template:SJTU-BioX-Shanghai/Notebook/Transformation/w10

pdark natural transformation

in the evening of 11th, Sep

6th liquid segregation of pdark-HR

pcpcG2 electro-transformation

in the evening of 11th Sep

5th liquid segregation of pcpcG2-HR

first desalination test of pcpcG2-HR

Sep. 7

Inoculated 15 wildtype & 15 pcpcG2-HR (use pcpcG2-HR from colony 2) at 10pm.

Sep. 9

Induced with green light at 10pm, added 22g/L NaCl & retinene, then took the first 6 samples (3 parallel samples for each).

Sep. 10

Cultivated with red light at 10am, then took the second 6 samples,

Took the third 6 samples at 2pm.

Took the fourth 6 samples at 10pm.

Sep. 11

Took the fifth 6 samples at 10am.

pdark-GFP electro-transformation

Sep. 8

We picked 1 colony (only 1 colony appeared) from the pdark-GFP plate and spread them on another BG11 plate in line to do solid segregation