Template:IONIS Paris/Notebook/09-09-15

E.coli DH5 alpha transformation

Transformation of competent cells with pSB1C3-GFP and pSB1C3-H/E (ratio 3:1, 1:1, 1:3) with 3 µl of plasmid.

Liquid cultures on plates from 08/09

Measurements of DO595 and DO405 (wtGFP) all the day long
Typical profile of colonies transformed with pDawn-HOKD under light exposition

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Continuous bacterial growth despite light exposure not expected


9 September 15