Team:Toulouse/Design
Device
Trap Construction
TPX Bag
Safety tests
TrApiColi has been designed in order to take into account ethical
reflection, safety and ease of use for beekeepers. The use of
genetically modified organisms in a field and more, associated with
edible products underlies both law enforcement and applying of
regulations and public perception account about it.
In this context, we designed a device being able to isolate our engineered bacteria from
the environment, but allowing its growth and metabolism. We found the
project of the iGEM Groningen 2012 team which used the polymer TPX® in
order to contain their bacteria separated of the meat [1]. Thus, we
decided to ask them TPX® characteristics and their supplier. We obtained
some samples of TPX®, enough to perform tests.
To check if our device can be safe and feasible, several tests have been performed:
Safety test: test the impermeability of bacteria through the bag of TPX®.
Permeability tests of butyric acid and formic acid through the bag of TPX®
Growth tests in TPX®: test if the strain E. coli BW25113 can grow in a TPX® bag (ie. in microaerobic conditions, without agitation and miming batch culture condition), as it would be in the field
Growth tests over 15 days
