Team:Toulouse/Design
Device
Trap Construction
TPX Bag
TrApiColi has been designed in order to take into account ethical
reflection, safety and ease of use for beekeepers. The use of
genetically modified organisms in a field and more, associated with
edible products underlies both law enforcement and applying of
regulations and public perception account about it.
In this context, we designed a device being able to isolate our engineered bacteria from
the environment, but allowing its growth and metabolism. We found the
project of the iGEM Groningen 2012 team which used the polymer TPX® in
order to contain their bacteria separated of the meat [1]. Thus, we
decided to ask them TPX® characteristics and their supplier. We obtained
some samples of TPX® by contacting the company MitsuiChemicals.
To check if our device can be safe and feasible, several tests have been performed:
Safety test: Impermeability of bacteria through the bag of TPX®
Gas diffusion tests: Permeability of butyric acid and formic acid through the bag of TPX®
Growth tests in TPX®: Culture of the strain E. coli BW25113 can grow in a TPX® bag (ie. in microaerobic conditions, without agitation and miming batch culture condition), as it would be in the field
Bacterial survival over 15 days in microaerobic condition
Carbone source test: choice of Carbon source to produce acids during 10 days
Acid toxicity on E. coli
