Team:KU Leuven/Research/Results

main

Research

Interlab

Modeling

Outreach

Future

Team

Notebook

main

Research

Interlab

Modeling

Outreach

Future

Team

Notebook

Results

Double Knockouts

The Centre of Microbial and Plant Genetics (KU Leuven) provided us with three E.coli K-12 strains, with each one representing the knockout for the genes tar, tsr or cheZ. The kanamycin cassette of the tar knock-out strain was removed by the enzyme flippase on pCP20. This excision was checked by PCR. Here the original knock-out strain of tar was used as a positive control and a band at 1232 base pairs is expected. If the cassette is removed, a band at 438 base pairs will be visible. Hereby, ten colonies were tested and all have lost their cassette (Figure 1).

Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Parts

A list of all used biobricks, the modifications we performed on them and the new ones we designed.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.

Back

Go back to the Research page.

Parts

A list of all used biobricks, the modifications we performed on them and the new ones we designed.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.

Results

Back

Go back to the main page.