House of Carbs

Parts

BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents	Backbone	Description	Function	Name in registry
GlgX	pSB1C3	Debranching enzyme (E.coli)	Coding sequence only	BBa_K1618025
GlgB	pSB1C3	Branching Enzyme (E.coli)	Coding sequence only	BBa_K1618000
MAO	pSB1C3	Acyl-transferase Candidate 1	Coding sequence only	BBa_K1618001
Rv3030	pSB1C3	Acylation candidate 2	Coding sequence only	BBa_K1618002
Rv3034c	pSB1C3	Acylation candidate 3	Coding sequence only	BBa_K1618003
Rv3037c	pSB1C3	Acylation candidate 4	Coding sequence only	BBa_K1618004

COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN

Part Contents	Backbone	Description	Function	Name in registry
P-Lac(R0010)_RBS(B0034)_GlgX(K1618026)	pSB1C3	Debranching enzyme expression construct	Removes glycogen branches	BBa_K1618026
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)	pSB1C3	Branching enzyme expression construct	Add glycogen branches	BBa_K1618022
P-Lac(R0010)_RBS(B0034)_MAO(K1618001)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618005
P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618007
P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618008
P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004)	pSB1C3	Acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618009
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001)	pSB1C3	Branching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002)	pSB1C3	Branching enzyme + acyl-transferase expression construct	)Transfers acyl groups to glycogen	BBa_K1618023
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003)	pSB1C3	Branching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618024
P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004)	pSB1C3	BBranching enzyme + acyl-transferase expression construct	Transfers acyl groups to glycogen	BBa_K1618027

COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (CODING SEQUNCES WERE FROM Coprococcus sp. L2-50 (DSM 16842)

Part Contents	Backbone	Description	Function	Name in registry
P-Lac(R0010)_RBS(B0034)_Buk	pSB1C3	Butyrate kinase expression contruct	Catalyzes the conversionbutyrate to/from butyryl	BBa_K1618012
P-Lac(R0010)_RBS(B0034)_PTB	pSB1C3	Phosphotransbutrylase expression construct	Catalyzes the conversion butyryl to/from butyryl CoA	BBa_K1618013
P-Lac(R0010)_RBS(B0034)_Buk_PTB	pSB1C3	Butyrate to butyryl CoA pathway	Catalyzes the conversion butyryl to/from butyryl CoA	BBa_K1618020
P-Lac(R0010)_RBS(B0034)_THL	pSB1C3	Thiolase expression construct	Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA	BBa_K1618014
P-Lac(R0010)_RBS(B0034)_CRO	pSB1C3	Crotonase expression construct	Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA	BBa_K1618015
P-Lac(R0010)_RBS(B0034)_BHBD	pSB1C3	β-hydroxybutyryl-CoA dehydrogenase expression	Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA	BBa_K1618016
P-Lac(R0010)_RBS(B0034)_BCD	pSB1C3	Butyryl-CoA dehydrogenase expression construct	Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA	BBa_K1618017
P-Lac(R0010)_RBS(B0034)_EFTb	pSB1C3	Electron transfer protein β expression construct	Catalyzes conversion of acetate to butyrate	BBa_K1618018
P-Lac(R0010)_RBS(B0034)_EFTa	pSB1C3	Electron transfer protein α expression construct	Catalyzes conversion of acetate to butyrate	BBa_K1618019
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD	pSB1C3	Acetyl-CoA to 3-crotonyl-CoA pathway	Catalyzes conversion of Acetyl-CoA to 3-crotonyl-CoA	--------
P-Lac(R0010)_RBS(B0034)_EFTa	pSB1C3	Crotonyl-CoA to Butyryl-coA pathway	Catalyzes conversion of Crotonyl-CoA to Butyryl-coA	-----------
P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa	pSB1C3	Acetyl-coA to Butryl-CoA pathway	Catalyzes conversion of Acetyl-coA to Butryl-CoA	BBa_K1618021
P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa	pSB1C3	Acetyl coA to butyrate complete biosynthetic pathway	Catalyzes conversion of Acetyl coA to butyrate	----------