Team:NTU-LIHPAO-Taiwan/Collaborations

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• Protocols
  • Preparation of Competent Cells (E.coli)
  • DNA Dissolution
  • Agarose Gel Electrophoresis
  • Gel Extraction
  • DNA Ligation
  • Plasmid DNA Extraction Using Alkaline Lysis Method (E.coli)
  • Transformation (E.coli)
  • Protein Extraction
  • SDS-PAGE
  • Western Blot
  • Immunostaining

Preparation of Competent Cells (E.coli)

Materials and Reagents :

1. Escherichia coli DH5α
2. LB broth
3. TB buffer
4. DMSO
5. Liquid nitrogen

Maintained by the iGEM team NTU-LIHPAO-Taiwan    ©2015 NTU-LIHPAO-Taiwan