This aptameric sequence is capable of recognising and binding specifically to lead II ions. This sequence, which works as a single stranded nucleic acid molecule, is capable of forming a G-quadruplex by interacting with lead ions (Pb2+) while disrupting a partially complementary duplex. For this competition purposes, its design was done in order to standardise it as a biobrick even though it is only functional as single stranded DNA. However, a primer was designed so that it can be retrieved from the biobrick cassette as a ssDNA molecule via an asymmetrical PCR.
BBa_K1636002: Linker
This linker was designed by the iGEM TecCEM team in order to retrieve single-stranded oligonucleotides when produced as single-stranded genetic material (e.g. plasmid). The linker should be flanking said oligonucleotides, and given the design of its sequence, it is able to form a hairpin-like secondary structure that allows the usage of a restriction enzyme (MlyI from New England BIolabs) with a blunt cut thereby releasing the oligos of interest.
BBa_K1636001: Primer for aptamer
This biobrick was designed in order to perform and asymmetrical PCR. The main aim of this part is the generation of high-copy oligos including the aptamer. Therefore, it is intimately related to the BBa_K1636000.
