All the work was done by students : wiki design , fundraising , policy and practices as all the cakes shown on social networks. In order to be more efficient, we decided to divide the work in little teams.
- Marie CECILLE proposed the original idea to work on Downy Mildew since the beginning of the brainstorming sessions.
- Jean DESCARPENTRIE and Emilie GOUNIN spent many hours reading publications and organizing little reunions around the scientific project
SPECIAL THANKS TO: - Camila PARROT member of INSERM / Université Bordeaux Segalen (Unit 869) NA: Natural and Artificial Regulation” (ARNA) led by Martin TEICHMANN, helped to optimize our purification of Curdlan.
- Nicolas BOISSET and Thomas FAVRAUD lead the yeast research before our lab-work and worked o the experiments during the summer
SPECIAL THANKS TO: - Derek McCUSKER, PhD Group Leader, CNRSIBGC - UMR 5095 (CNRS-UBS) helped us by explaining the homologous repair to express the FKS1 gene and on the design of primers for this gene. - Denis DUPUY PhD Group Leader of INSERM/Université Bordeaux Segalen (U869); ARN: Régulations naturelle et artificielle" (ARNA) ; our supervisor ; led us for the Gibson chew back assembly technique - Jonathan MILLET, Denis DUPUY’s PhD student, gave us some advices for PCR troubleshooting.
- Savandara BESSE , Marjorie XXX and Hiba BENMOHAMED worked hard all year writing e-mails and contacting companies for sponsoring. - Lily BOURGOIN and Edouard TOURDOT and Cassandra XXX made the sponsoring plaquette and flyers which were distributed during our different events, including wine congresses - Jean-Rémy XXX helped with the paperwork to be done for help from the Aquitaine region and the Bordeaux University
- Hiba BENMOHAMED - On yeast work, - Lionel BEAUREPAIRE, INSERM engineer, gave many advices on clonage. - To test Curdlan, we have contacted Anthony Bellee PhD student attached to INRA , SAVE unit : "Santé et agroécologie du vigneoble"who helped us a lot and inform us about how Downy mildew and plant defenses. He let one of our teammate manipulate and realize the test. Michel Hernould Professor Bordeaux University, UMR 1332 Fruit Biology and Pathology INRA Bordeaux .He gave us a precious advices about plant, and permits to the team to meet Anthony Bellee in order to test the effect of Curdlan in grapevine. - Concerning our wiki, it was based on Evry's 2014 team wiki. We decided to adapt their code for our wiki. We also received a great help of Nacim BENALI, student in informatics, for the design of the wiki. - The comics were designed by Caroline GAGNANT, one of our classmates. - On Ethics section, M. Jacques FAUCHER’s interview helped us to understand the utility of ethics committees.
During our entire project, many people helped us to accomplish our project Cur’d Vine. First, we want to thank the European Institute of Chemistry and Biology for hosting us all the year and during our internships. Denis DUPUY, our supervisor who gives us many judicious advices and Jonathan MILLET who succeeded to coexist with many team members of iGEM Bordeaux during all this summer. Our team had to use several machines which not belong to our lab. That’s why we had to collaborate many times with the neighbor labs. We hope that they enjoyed our cake as gift. ;) Thanks to: - Aurore DE RACHE, Amina BEDRAT, Nassima GUEDDOUDA and Oscar MENDOZA from Jean-Louis Mergny’s Group to lend us their spectrophometer, their NanoDrop instruments and their Fluorescence Reader. - Martin TEICHMANN's group to lend us incubators and the pHmeter - Natacha PEREBASKINE from Axel INNIS’ group, for the safety formation and for the lending of their Nanodrop instruments - Myriam MEDERIC for her hard work - Camila PARROT for her daily support Moreover, we want to thank Didier THORAVAL, teacher and researcher of "Laboratoire de Biogenèse Membranaire" gave us many resources for our work on yeast. Without this help, yeast work will never start. Also, we are grateful that M. McIntosh helped us by answering to our questions on Curdlan. We would also like to thank CAP SCIENCES (the Bordeaux sciences musuem) for hosting the show and especially Nathalie CAPLET and Sébastien CURSAN for the organization and the communication about this event! Finally, thanks to have filmed the various presentations.
At the end, thanks to all other people who supported our project on social media (Facebook, Twitter, Linkedin and Youtube). Your interest had always touched us and we hope that we will support again for 2016 iGEM competition!
Team Registration. ✔ Complete Judging form. ✔ Create a Team Wiki. ✔ Present a poster and a talk at the iGEM Jamboree. ✔ Create a page Team on the wiki. ✔ Look at our Page Team Page Describe Attribution of each aspect of your project. ✔ Look at our Attributions Page Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry. ✔ Look at our Biobricks Page
Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. ✔ Submit this new part to the iGEM Parts Registry. ✔ Demonstrate how your team has identified, investigated and addressed one or more of these issues in the context of your project. ✔
Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the design and/or execution of your project OR demonstrate an innovative human practices activity that relates to your project. ✔ Help any registered iGEM team from another school or institution. ✔ Improve the function OR characterization of an previously existing BioBrick Part or Device. ✔ Demonstrate a functional prototype of your project. Your prototype can derive from a previous project (that was not demonstrated to work) by your team or by another team. ✔