Template:IONIS Paris/Notebook/11-09-15

Digestion

Mix digestion preparation:

Tube	VVD YC	VVD YN
H2O MQ	1 µL	1 µL
Buffer 2.1	2 µL	2 µL
DNA	15 µL	15 µL
Enzyme 1	EcoRI 1 µL	XbaI 1 µL
Enzyme 2	SpeI 1 µL	PstI 1 µL

37°C, 1h heat kill: 65°C, 20min
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11 Sept. 15