Team:Chalmers-Gothenburg/BioBricks
Contents
BioBricks
1. BBa_K1321000: pTEF1-pSUC2
The high expression pTEF1 promoter connected to pSUC2 promoter. Allows induced high expression of downstream gene at low glucose concentrations.
2. BBa_K1321001: STE2MAM2
Fusion protein. The non-cytoplasmic N-terminal signal peptide from Saccharomyces cerevisiae and Pheromone P-factor receptor (GPCR) from schizosaccharomyces pombe. Allows S.cerevisiae to detect the Pheromone P-factor through the Pheromone pathway.
3. BBa_K1321002: RecA
Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair
4. BBa_K1321004: pTPI1
promoter to TPI1.
5. BBa_K1321005: Ssb
From Deinococcus Radiodurans Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair