Team:Oxford/Synbiota
Synbiota
We were awarded trailblazer scholarship by Synbiota, a startup company co-founded by iGEM alumni, and had a chance to test their prototype Rapid DNA Prototyping (RDP) assembly standard including exclusive iGEM kit and RDP part designing software.
RDP is a modular approach to synthetic biology that is biobrick compatible, and somewhat similar to standard biobrick approach in a sense that DNA templates can create new RDP parts through PCR. However, each RDP part is defined by each promoter, RBS, coding region, or terminator. Series of RDP parts can be assembled using magnetic anchor under two hours.
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RDP Standard requires addition of two unique sticky ends (X and Z) and their complements (X’ and Z’) onto each part, allowing sequential assembly from promoter to terminator.
We have designed two customised RDP parts, pLsr and T4Holin. These RDP parts were designed using GEntle software(https://gentle-beta.synbiota.com/) to contain X-Z’ or Z-X’ sticky ends. Synbiota has provided us with physical RDP parts of pLse and T4Holin, as well as primers to amplify them through PCR. Wetlab notebooks of Synbiota specific PCR can be found here