University of Virginia iGEM 2015
IPTG Promoter+RBS+EIIA+T
BBa_XXXXX
BioBrick BBa_1600001serves to increase the uptake of glucose by the cell. This extra glucose will then be polymerized by one of our following BioBrick devices (BBa_ . This composite BioBrick is composed of BBa_K215000 (IPTG promoter), BBa+R0034 (RBS) and BBa_K1508003 (EIIA plus promoter).
Promoter+RBS+GlgC+TT
BioBrick BBa_1600002 is one of our polymerization circuits and serves to polymerize glucose into the polysaccharide glycogen in order to increase sugar absorption time thus minimizing the probability of glycemic spikes. This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K118018 (RBS and Glgc), and BBa_B0015 (double terminator).
Promoter+RBS+SacB+TT
BioBrick BBa_1600003 is another of our polymerization circuits and is important not only for polymerization of levan but also acts as our kill switch which is activated once there is greater than 5% of fructose in the cell. This serves as an important aspect of our safety factor since this kill switch is responsible for the lysing of the cell to prevent colonization of antibiotic resistant bacteria in the gut. This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K322921 (RBS and SacB) and BBa_B0015 (double terminator).
Promoter+RBS+GlgC+RBS+SacB+TT
BioBrick BBa_1600004 is our composite polymerization circuit combining the functions of BBa_1600002 and BBa_1600003. This BioBrick polymerizes glucose into glycogen, polymerizes levan from fructose, and possesses the kill switch responsible for lysing the cell. This composite BioBrick is composed of BBa_J23110 (promoter), BBa_K118018(RBS and Glgc), BBa_K322921 (RBS and SacB) and BBa_B0015 (double terminator).
EIIBC
