Team:Penn/Notebook

University of Pennsylvania iGEM

Content Expander

expanding content since 2014

robsawyer.me

6/8/2015

Aims for the day:

  1. Run a gel after digestion of lacZ and pDawn
  2. Gel extraction & digest cleanup
  3. Ligation
  4. Transformation

 

Accomplishments:

  1. Ran a gel after digestion
  2. Performed gel extraction and digestion cleanup
  3. Measured concentrations of pDawn and lacZ with nanodrop

 

Aims for tomorrow:

  1. PCR clean-up for new PCR of lacZ
  2. Digestion of lacZ and pDawn with new Nde1 and BamHI
  3. Digest clean-up
  4. Ligation of lacZ and pDawn
  5. Lux box transformation into E. coli

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