Team:Warwick/Modeling
Modelling
Modelling is a key part of synthetic biology. Some experiments take too long, are far too expensive, or the information required just can’t be found via lab work. This is where modelling comes in. We take information from the biologist, construct a theoretical framework, and then feed back to people in the lab about what they should do.
In our project we have come up with a number of models, ranging from cell division to equilibrium reactions.
Click on the images below to explore more.
Binding Affinity Modelling
A big problem biologists have is the uncertainty of bonding of cells, especially in our design where zinc fingers need to bind to cells. Therefore it is important to come up with a model which can calculate the number of cell and zinc finger binding sites required for a given output. Here we discussed how the zinc fingers bind to the glass slides and the association and disassociation constants which are factors in the number of cell that fully bind.
DNA Origami Glue Modelling
This page contains the model which discusses the use of DNA as an oligonucleotide adhesive using to create 2D and 3D structures and shapes.
Cell Growth Interactions
This section deals with the problem of what would happen to the previous shapes as time passes and cells grow.
Tetrahedron Construction
This shows how a 3D structure could be created from the minimum amount of DNA using tetrahedrons as a base to build from. Cells would then be bound to the outside.
Cube Construction
Similar to the previous, this uses cubes to create 3D shapes and discusses the minimum size need for DNA origami shapes.
3D Lithography
This discusses the different possibilities of the creation of 3D structures and shapes in a general sense.