Team:CGU Taiwan/Notebook

Description

Yeast With IL-8 Receptor

Toehold Switch As RNA Senor

Prototype Design

GPCR

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol

Strains	260/280	260/230	C(ng/μl)
Far1∆::KANMX strain	1.62	0.97	44.7

1. Design of primers

					Tm (℃)	GC (%)	Nmole	μl for 100μM
						55	21.25	212.50
						41	17.12	171.20

GPCR

2015.7.1

Operator: Wan yun, Jinting , Jinye Goal: 1. Extraction of gDNA of Far1∆::KANMX strain 2. Detection the concentration of fast extracted gDNA of ∆Far1 strain 3. PCR gDNA of Far1∆ ::KANMX 4. Electrophoresis to check PCR product Experiment steps: < Extraction of gDNA of Far1∆::KANMX strain> Consult the protocol

Strains	260/280	260/230	C(ng/μl)
Far1∆::KANMX strain	1.62	0.97	44.7

1. Design of primers

					Tm (℃)	GC (%)	Nmole	μl for 100μM
						55	21.25	212.50
						41	17.12	171.20