Team:HUST-China/Achievements
Achivements
Judging form
For the Bronze Medal:
√ Register for iGEM, have a great summer, and plan to have a good time at the Giant Jamboree.
√ Successfully complete this year‘s judging form.
√ Create and share a Description of the team's project using the iGEM wiki, and document the team's parts using the Registry of Standard Biological Parts.
√ Prepare a poster and a talk for the iGEM Jamboree.
√ Create a page on our wiki with clear attribution of each aspect of our project, and clearly attribute work done by ourselves.
√ Document more than one new standard BioBrick Parts or Devices central to our project and submit these parts to the iGEM Registry.
| Type | Part Number | Notes |
|---|---|---|
| Coding | BBa_K1592000 | LIP2 |
| Coding | BBa_K1592001 | Mcfp3 |
| Coding | BBa_K1592002 | YLcwp3 |
| Coding | BBa_K1592003 | LIP2+Mcfp3 |
| Coding | BBa_K1592004 | Php4d |
| Coding | BBa_K1592005 | BD-CRY2 |
| Coding | BBa_K1592006 | AD-CIB1 |
As for the Slier Medal:
√ Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected.
√ Document the characterization of new parts in the Main Page section of the Registry entry for that Part/Device, and submit these new parts to the iGEM Parts Registry.
√ In our Human Practice this year, we majorly concentrated on promoting the concept of synthetic biology through interdisciplinary communication and field practice at fishery industry. We communicated with Professor Zheng Junjie from Civil Engineering College, visited Zhejiang Marine Fisheries Research Institute(MFRI) and took a field trip to Zhoushan Fishery.
Click here to see more details.
| Type | Part Number | Notes |
|---|---|---|
| Coding | BBa_K1592007 | His-Si-tag1 |
| Coding | BBa_K1592008 | His-Si-tag2 |
| Coding | BBa_K1592009 | His-Si-tag3 |
| Coding | BBa_K1592010 | Si-tag12 |
| Coding | BBa_K1592011 | Si-tag23 |
| Coding | BBa_K1592012 | Si-tag13 |
| Coding | BBa_K1592013 | His-Si-tag1L3 |
| Coding | BBa_K1592014 | Si-tag123 |
| Coding | BBa_K1592015 | CRY2 |
| Coding | BBa_K1592016 | CIB1 |
| Coding | BBa_K1592017 | XPR2+Mcfp3 |
| Coding | BBa_K1592018 | Pgal1+rox1+cyc1 |
| Coding | BBa_K1592019 | Panb1+XPR2 pre-Mcfp3 |
As for the Gold Medal:
√ After deciding the main application of our kit we want to carry out, we visited Professor Weiding Wang, an expert in aquafarm and artificial reefs form Zhejiang Marine Fisheries Research Institute (MFRI) and issued questionnaires and conducted interviews among local fishermen and practitioners. During this process, we gained a better perception of the development of synthetic biology in China, and also learned valuable practical knowledge from experts, public.
Click here to see more details.
√ This summer, we helped WHU team constructing a standardized plasmid C1-pSB1C3 when they met difficulties. And we helped WHU-China team and HZAU-China lyophilize the samples before shipment as their lyophilizer cannot handle the 96well plate.
√ We found two SpeI illegal sites in Part BBa_K191007, so we removed that by site-directed mutagenesis and created 3 different mutants of Part BBa_K191007. We inserted RBS + GFP for test and verify, but unfortunately we can’t get the competitive inhibitor of tryptophan in the formation of the specific Apo repressor. Moreover, we also documented the Pgal1 promoter (BBa_K1144001) and Gal4 binding domain (BBa_K801032).
| Type | Part Number | Notes |
|---|---|---|
| Regulatory | BBa_K1592020 | Ptrp mutant1 |
| Regulatory | BBa_K1592021 | Ptrp mutant2 |
| Regulatory | BBa_K1592022 | Ptrp mutant3 |
| Composite | BBa_K1592023 | Ptrp mutant1+RBS+GFP |
| Composite | BBa_K1592024 | Ptrp mutant2+RBS+GFP |
| Composite | BBa_K1592025 | Ptrp mutant3+RBS+GFP |