Team:Bordeaux/Yeast results

IGEM Bordeaux 2015





CHOICE OF MATERIALS

FKS1 gene
FKS1 gene codes a D-glucan synthase.

pYES2 plasmid

The plasmid used is the pYES2 plasmid provided by M. Didier Thoraval (membrane biogenesis laboratory). It contains an inducible promoter to galactose (GAL1) and a selection gene URA3.

Saccharomyces cerevisiae strain

All results were obtained with haploid Lnvsc1 strain of Saccharomyces cerevisiae. Its genotype is Leu / Trp / Ura- / Hist- mat α. This genotype allows us an easy selection of transformed cells.