Team:NRP-UEA-Norwich/Parts
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching enzyme (E. coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E. coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyl-transferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acylation candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acylation candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acylation candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618026) | pSB1C3 | Debranching enzyme expression construct | Removes glycogen branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching enzyme expression construct | Add glycogen branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_MAO(K1618001) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | BBranching enzyme + acyl-transferase expression construct | Transfers acyl groups to glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate kinase expression contruct | Catalyzes the conversionbutyrate to/from butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase expression construct | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to butyryl CoA pathway | Catalyzes the conversion butyryl to/from butyryl CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase expression construct | Catalyzes the conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase expression construct | Catalyzes conversion of3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA dehydrogenase expression | Catalyzes conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA dehydrogenase expression construct | Catalyzes conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron transfer protein β expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron transfer protein α expression construct | Catalyzes conversion of acetate to butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-coA to Butryl-CoA pathway | Catalyzes conversion of Acetyl-coA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl coA to butyrate complete biosynthetic pathway | Catalyzes conversion of Acetyl coA to butyrate | BBa_K1618046 |
COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 expression construct | Transfers acyl groups to starch | BBa_K1618033 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 expression construct | Transfers acyl groups to starch | BBa_K1618034 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 expression construct | Transfers acyl groups to starch | BBa_K1618035 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 expression construct | Transfers acyl groups to starch | BBa_K1618036 |
| P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 1 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618029 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 2 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618030 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 3 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618031 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acylation candidate 4 -fused to GFP -expression construct | Check localization in plastid of candidate gene | BBa_K1618032 |
| 35s Terminator | pSB1C3** | Eukaryotic terminator from the 35s gene (Cauliflower Mosaic Virus) | terminates transcription in plant cells | BBa_K1618037 |
| Chloroplast Transit Peptide | pSB1C3*** | Synthetic plastid transit peptide form the consensus of multiple RuBisCo Small Subunit genes | In an N-terminal fusion, this peptide directs proteins to the plant plastid. | BBa_K1618028 |
* This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcritional unit flipper) made by the NRP UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium-tumefaciens mediates trasnfection.
** This was convered to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (terminator flipper) made by the NRP UEA 2014 team.
*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.
