Team:HUST-China/Notebook
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Details
Standalization
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Week 6 8.3-8.9
PCR: More details, protocol WangJie Liu and JunJie Xu
Restriction Enzyme Digestion
WangJie Liu and JunJie Xu
DNA ligation&Transformation
WangJie Liu and JunJie Xu
Successfully align the sequencing results with the objective sequences:
Colony PCR
WangJie Liu and JunJie Xu
Extract the plasmid,Conserving.
Week 7 8.10-8.16
PCR:
More details, protocol
WangJie Liu
Restriction Enzyme Digestion
WangJie Liu
DNA ligation&Transformation
WangJie Liu
Colony PCR
Successfully align the sequencing results with the objective sequences:
WangJie Liu
Extract the plasmid,Conserving.
Week 9 8.24-8.30
Week 10 8.31-9.6
Successfully align the sequencing results with the objective sequences
Standalization of BD-CRY2 and AD-CIB1 finished.
Successfully align the sequencing results with the objective sequences
Standalization of CRY2 and CIB1 finished.
Light Control
Week 1 6.29-7.5
Week 2 7.6-7.12
Successfully align the sequencing results with the objective sequences of Pgal1 and CYC_TT.
Extract the plasmid.
Conserving.
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Successfully align the sequencing results with the objective sequences
Digestion Test
Guozhao Wu
Week 6 8.3-8.9
Successfully align the sequencing results with the objective sequences
Pgal-ROX1-CYC_TT circuit was constructed.
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
viscous parts
Week 1 6.29-7.5
Week 2 7.6-7.12
PCR:
More details, protocol
Wang Xi and JunJie Xu
Restriction Enzyme Digestion
Wang Xi and JunJie Xu
DNA ligation&Transformation
Wang Xi and JunJie Xu
Colony PCR
Successfully align the sequencing results with the objective sequences:
Wang Xi and JunJie Xu
Extract the plasmid,Conserving
Week 3 7.13-7.19
PCR:
More details, protocol
ZhangYu Cheng
Restriction Enzyme Digestion
ZhangYu Cheng
DNA ligation&Transformation
ZhangYu Cheng
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
Extract the plasmid,Conserving.
Week 4 7.20-7.26
PCR:
More details, protocol
Wang Xi and JunJie Xu
Restriction Enzyme Digestion
Wang Xi and JunJie Xu
DNA ligation&Transformation
Wang Xi and ZhangYu Cheng
Colony PCR
Wang Xi and JunJie Xu
Successfully align the sequencing results with the objective sequences:
Extract the plasmid,Conserving.
Tramsformation to yeast
Lei Yin
Genome extract and PCR test
Lei Yin
Positive strain conservation
Week 5 7.27-8.2
PCR:
More details, protocol
ZhangYu Cheng
Restriction Enzyme Digestion
ZhangYu Cheng
DNA ligation&Transformation
ZhangYu Cheng
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
Extract the plasmid,Conserving.
Trasnsform to yeast
Genome extract and PCR test
Lei Yin
Positive strain conservation
Week 6 8.3-8.9
PCR:
More details, protocol
ZhangYu Cheng
Restriction Enzyme Digestion
ZhangYu Cheng
DNA ligation&Transformation
ZhangYu Cheng
Colony PCR
ZhangYu Cheng
Successfully align the sequencing results with the objective sequences:
Extract the plasmid,Conserving.
Trasnsform to yeast
Lei Yin
Genome extract and PCR test
Lei Yin
Positive strain conservation
Week 7 8.10-8.16
SDS-PAGE
Lei Yin
Immunoflourecense
ZhangYu Cheng
Verification of the secreting Mcfp-3 (Coomassie blue staining)
ZhangYu Cheng
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
Improvement
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Week 6 8.3-8.9
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
Consolidation
Week 1 6.29-7.5
Week 2 7.6-7.12
Week 3 7.13-7.19
Week 4 7.20-7.26
Week 5 7.27-8.2
Week 6 8.3-8.9
Week 7 8.10-8.16
Week 8 8.17-8.23
Week 9 8.24-8.30
Week 10 8.31-9.6
