Team:NRP-UEA-Norwich/Collaborations/Manchester
Manchester-Graz Collaboration
The Manchester-Graz team have developed an expression system designed to regulate single and multi-gene pathways for an intestine expression. For controlling a wide range of pathways it is designed in a flexible and modular manner. They tested the production of butyrate in the gut. The pathway was incorporated into the expression system model to observe the expression of butyrate under the control of the developed system. The model generated helped us to understand how the system is dealing with pathways that consist of several enzymes at an intestine level.
The system consists of two quorum sensing (QS) systems EsaR/I and CepR/I. The EsaR/I system belongs to the plant pathogen Pantoea stewartii. The second QS-System, CepR/I, belongs to the opportunistic pathogen Burkholderia cenocepacia. For details about the system and the model please look into: https://2015.igem.org/Team:Manchester-Graz/modelling.
Figure 1: Overview of the reduced pathway. Coenzyme A (Co A) is recycled in the pathway by the butyryl CoA-acetyl CoA-tranferase.
Butyrate is converted starting from two Acetyl CoAs over several steps to Butyryl CoA. In the last step the Coenzyme A is transferred to Acetate, producing Acetyl CoA and Butyrate (1). For simplicity, the pathway was reduced to some essential parts and steps in the pathway. Acetate is converted to Acetyl CoA. The steps to Butyryl CoA are reduced to one step. Coenzyme A is recycled in the last step to Butyrate and can be reused to produce Acetyl CoA (Figure 1).
Glyco2D Works!
When we increase the initial percentage of random acylation, the total number of modified glucoses increased. We showed a great linear correlation between both (see figure 3)
Figure 3: A graph showing the results from Glyco2D acylation simulation.
