Team:NRP-UEA-Norwich/Parts
Parts
BASIC PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| GlgX | pSB1C3 | Debranching Enzyme (E. coli) | Coding sequence only | BBa_K1618025 |
| GlgB | pSB1C3 | Branching Enzyme (E. coli) | Coding sequence only | BBa_K1618000 |
| MAO | pSB1C3 | Acyltransferase Candidate 1 | Coding sequence only | BBa_K1618001 |
| Rv3030 | pSB1C3 | Acyltransferase Candidate 2 | Coding sequence only | BBa_K1618002 |
| Rv3034c | pSB1C3 | Acyltransferase Candidate 3 | Coding sequence only | BBa_K1618003 |
| Rv3037c | pSB1C3 | Acyltransferase Candidate 4 | Coding sequence only | BBa_K1618004 |
COMPOUND PARTS FOR ENGINEERING BACTERIAL GLYCOGEN
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_GlgX(K1618025) | pSB1C3 | Debranching Enzyme Expression Construct | Removes Glycogen Branches | BBa_K1618026 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000) | pSB1C3 | Branching Enzyme Expression Construct | Adds Glycogen Branches | BBa_K1618022 |
| P-Lac(R0010)_RBS(B0034)_MAO(K1618001) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618005 |
| P-Lac(R0010)_RBS(B0034)_Rv3030(K1618002) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618007 |
| P-Lac(R0010)_RBS(B0034)_Rv3034c(K1618003) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618008 |
| P-Lac(R0010)_RBS(B0034)_Rv3037c(K1618004) | pSB1C3 | Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618009 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3030(K1618002) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618023 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3034c(K1618003) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618024 |
| P-Lac(R0010)_RBS(B0034)_GlgB(K1618000)_Rv3037c(K1618004) | pSB1C3 | Branching Enzyme + Acyltransferase Expression Construct | Transfers Acyl Groups to Glycogen | BBa_K1618027 |
COMPOUND PARTS FROM THE BUTYRATE BIOSYNTHESIS PATHWAY (Coding sequences from Coprococcus sp. L2-50 -DSM 16842-)
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-Lac(R0010)_RBS(B0034)_Buk | pSB1C3 | Butyrate Kinase Expression Contruct | Catalyzes the Conversion of Butyrate to/from Butyryl | BBa_K1618012 |
| P-Lac(R0010)_RBS(B0034)_PTB | pSB1C3 | Phosphotransbutrylase Expression Construct | Catalyzes the Conversion of Butyryl to/from Butyryl-CoA | BBa_K1618013 |
| P-Lac(R0010)_RBS(B0034)_Buk_PTB | pSB1C3 | Butyrate to Butyryl-CoA Pathway | Catalyzes the Conversion of Butyryl to/from Butyryl-CoA | BBa_K1618020 |
| P-Lac(R0010)_RBS(B0034)_THL | pSB1C3 | Thiolase Expression Construct | Catalyzes the Conversion of Acetyl-CoA to Acetoacetyl-CoA | BBa_K1618014 |
| P-Lac(R0010)_RBS(B0034)_CRO | pSB1C3 | Crotonase Expression Construct | Catalyzes the Conversion of 3-hydroxybutyryl-CoA to Crotonyl-CoA | BBa_K1618015 |
| P-Lac(R0010)_RBS(B0034)_BHBD | pSB1C3 | β-hydroxybutyryl-CoA Dehydrogenase Expression | Catalyzes the Conversion of Acetoacetyl-CoA to 3-hydroxybutyryl-CoA | BBa_K1618016 |
| P-Lac(R0010)_RBS(B0034)_BCD | pSB1C3 | Butyryl-CoA Dehydrogenase Expression Construct | Catalyzes the Conversion of Crotonyl-CoA to Butyryl-CoA | BBa_K1618017 |
| P-Lac(R0010)_RBS(B0034)_EFTb | pSB1C3 | Electron Transfer Protein β Expression Construct | Catalyzes Conversion of acetate to butyrate | BBa_K1618018 |
| P-Lac(R0010)_RBS(B0034)_EFTa | pSB1C3 | Electron transfer protein α Expression Construct | Catalyzes Conversion of Acetate to Butyrate | BBa_K1618019 |
| P-Lac(R0010)_RBS(B0034)_THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butryl-CoA Pathway | Catalyzes Conversion of Acetyl-CoA to Butryl-CoA | BBa_K1618021 |
| P-Lac(R0010)_RBS(B0034)_Buk-PTB-THL-CRO-BHBD-BCD-EFTb-EFTa | pSB1C3 | Acetyl-CoA to Butyrate Complete Biosynthetic Pathway | Catalyzes Conversion of Acetyl-CoA to Butyrate | BBa_K1618046 |
COMPOUND PARTS FOR ENGINEERING PLANT STARCH
| Part Contents | Backbone | Description | Function | Name in registry |
|---|---|---|---|---|
| P-35s(K1467101)_TP(K1618037):MAO(K1618001)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 1 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618033 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 2 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618034 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 3 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618035 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 4 Expression Construct | Transfers Acyl Groups to Starch | BBa_K1618036 |
| P-35s(K1467101)_TP(K1618037):MAO(K1618001):GFP(K1467204)_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 1 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618029 |
| P-35s(K1467101)_TP(K1618037):Rv3030(K1618002):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 2 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618030 |
| P-35s(K1467101)_TP(K1618037):Rv3034c(K1618003):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 3 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618031 |
| P-35s(K1467101)_TP(K1618037):Rv3037c(K1618004):GFP(K1467204_T-35s(BBa_K1618037) | pSB1C3* | Acyltransferase Candidate 4 -Fused to GFP -Expression Construct | Check Localization in Plastid of Candidate Gene | BBa_K1618032 |
| 35s Terminator | pSB1C3** | Eukaryotic Terminator from the 35s Gene (Cauliflower Mosaic Virus) | Terminates Transcription in Plant Cells | BBa_K1618037 |
| Chloroplast Transit Peptide | pSB1C3*** | Synthetic Plastid Transit Peptide From the Consensus of Multiple RuBisCo Small Subunit Genes | In an N-terminal Fusion, this Peptide Directs Proteins to the Plant Plastid. | BBa_K1618028 |
* This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467400 (complete transcriptional unit flipper) made by the NRP-UEA 2014 team. These parts were tested by cloning into a binary plasmid vector backbone prior to delivery to plants leaves using Agrobacterium tumefaciens mediated transfection.
** This was converted to a standard BioBrick by cloning into the Mo-Flipper Plasmid K1467300 (Terminator Flipper) made by the NRP-UEA 2014 team.
*** This is assembled in a Golden Gate Universal Acceptor Plasmid (pUAP1) in the pSB1C3 backbone, according to RFC 106. It is a Golden Gate part in the plant standard syntax, not a BioBrick.
