Part:BBa_K1864000

Part Name: CSBV-RdRP

Part type: Coding

Creator :Ruicheng Dai

Sequence :BBa_K1864000 Part-only sequence (876 bp)

Short Description:Our part is CSBV's RdRP,RdRP gene cloned into the L4440 vector,and transformed into E.coli strain HT115 to express dsRdRP.

Long Description: RNA-dependent RNA polymerase (RdRp),(RDR),or RNA replicase, is an enzyme that catalyzes the replication of RNA from an RNA template. This is in contrast to a typical DNA-dependent RNA polymerase, which catalyzes the transcription of RNA from a DNA template. CSBV RNA-dependent RNA polymerase (CSBV-RdRp) is an essential protein encoded in the genomes of viruses with no DNA stage that have sense negative RNA.

Part:BBa_K1864001

Part Name: T7 RNA polymerase

Part type: Coding

Creator :Ruicheng Dai

Sequence :BBa_K1864001 Part-only sequence (2652 bp)

Short Description:T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA in the 5'→ 3' direction.

Long Description: RNA-dependent RNA polymerase (RdRp),(RDR),or RNA replicase, is an enzyme that catalyzes the replication of RNA from an RNA template. This is in contrast to a typical DNA-dependent RNA polymerase, which catalyzes the transcription of RNA from a DNA template. CSBV RNA-dependent RNA polymerase (CSBV-RdRp) is an essential protein encoded in the genomes of viruses with no DNA stage that have sense negative RNA.