Team:Technion Israel/Lab Notebook/Protocols

Team: Technion 2015

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Protocols

Gel Preparation

Sigma-Aldrich Miniprep

Primer Dilution

CIP

Gibson Assembly

Restriction

UV Gel Viewing

Gel Extraction

Transformation

  1. E.coli
  2. B.subtilis

Colony PCR

  1. E. coli
  2. B. subtilis

Ligation

Nanodrop Use

Bacillus Secretion Verification

3ɑ-HSD Activity Verification Protocol

Extracellular NADPH Estimation

Comparison of Lysis Procedures

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