Template:Team:Groningen/CONTENT/EXPERIMENTS/Colony PCR thrC locus 2 (th18)

00:00, 4 September 2015 - 00:00, 4 September 2015

Colonies from the transformation were checked with a colony PCR. Herefore the following primers were used.


IGEM thrC forward AATTCATGTAAAAGATGAGGTTGGTTCATT


iGEM erm-thrC rev 2 AGGAAGTTAAAGGAGCTCGAATTAATTCC


expected bands around 2500 bp. Colony 5 showed the correct band on the agarose gel after the colony PCR. This colony was grown on LB with Ampicillin overnight at 37°C

Marieke Mulder