iGEM Kent 2015
Notebook
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June
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1
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2
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3
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4
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5
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6
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7
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8
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9
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10
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11
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12
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13
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14
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15
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16
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17
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18
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19
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20
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21
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22
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23
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24
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25
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26
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27
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28
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29
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30
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Contents
Week 1
Week 2
Week 1
Day 1 Wet lab 22/06/15
Made LB plates
Made LB broth
TIPS autoclaved
Day 2 Wet lab 23/06/15
Set up Top10 overnight, VS45 overnight, US348 overnight
Day 3 Wet lab 24/06/15
Filter sterilise the buffer
250ml no antibiotic broth, put top10 cells in
Incubate until optical density is 0.6
Miniprep PSB1C3 plasmid
Day 4 Wet lab 25/06/15
Transformation
Prepare SBC media
Digest of PSB1c3 from MS348
Day 5 Wet lab 26/06/15
Calculated competent cell efficiency
Agarose gel formation
Gel electrophoresis
Week 2
Day 6 Wet lab 29/06/15
Transforming linear PSBC13