Team:Kent/Notebook


iGEM Kent 2015

Notebook

June
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Contents

Week 1
Week 2

Week 1

Day 1 Wet lab 22/06/15

  • Made LB plates
  • Made LB broth
  • TIPS autoclaved
  • Day 2 Wet lab 23/06/15

  • Set up Top10 overnight, VS45 overnight, US348 overnight
  • Day 3 Wet lab 24/06/15

  • Filter sterilise the buffer
  • 250ml no antibiotic broth, put top10 cells in
  • Incubate until optical density is 0.6
  • Miniprep PSB1C3 plasmid
  • Day 4 Wet lab 25/06/15

  • Transformation
  • Prepare SBC media
  • Digest of PSB1c3 from MS348
  • Day 5 Wet lab 26/06/15

  • Calculated competent cell efficiency
  • Agarose gel formation
  • Gel electrophoresis
  • Week 2

    Day 6 Wet lab 29/06/15

    Transforming linear PSBC13