Team:UFMG Brazil/Results

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Results

DNA coding sequence of Mouse interferon-beta (IFN-beta) linked to signal peptide that allow protein secretion and latency-associated peptide (LAP) that possibilite IFN activation only in inflammatory site was designed with optimized codons for expression in Leishmania host (Figure 1).

Figure 1 - LAP-IFN-beta synthetic gene synthetized. Highlighted in green is signal peptide to secretion and highlighted in purple is LAP sequence.

The final sequence was synthesized by IDT (California, USA) in pUC_IDT standard vector with resistance to ampicillin. E. coli strain DH10b/TOP10 was transformed with the plasmid and resistant colonies were observed after incubation by 16 hours at 37 oC (Figure 2).