Team:Warwick/Modelling6
Cell Growth and Interactions between multiple Cell types
There was an inherent issue with a previous model in that the structure of the created shape would change as cells grew. We needed to model how they would grow and how the placement of the cells in the start would affect growth.
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Stochastic Modelling for Cell Growth
We wanted to understand how the placement of cells changes as time progresses. Cells divide, roughly in the same direction in which they were formed. To model cell division we imagined them situated on a Cartesian coordinate system, and division to place on a polar coordinate system with the original cell as the origin. The population count is modelled with the Gompertz function. To decide which direction the division occur we used two criteria:1. It must not overlap other cells or DNA
2. The probability is weighted by a normal distribution with the peak in the same direction as the previous generational division
The user inputs the DNA structure, the standard deviation for the normal distribution, and the maximum number of cells it should model. It then outputs an animation of the cell community as it develops.
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Simple Exponential Model and Gompertz Function
The equation on the right is the Gompertz function which is similar to the first model expect the growth of the cells decreases as the number of cells reaches a limit. This is a much better model as it takes into account the upper limit of cell concentration possible in a given volume.
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Comparing the two
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Cell growth with Interactions
Our idea of forcing different cell types to live together in close vicinity will alter this model however. Taking for example forcing 3 cell types together;
A higher concentration of cell type A could lead to a reduction in cell type B, or an increase in B could increase C.
It is important for cells to have a starting point where they are all stuck together instead of just 3 different cell types being put into a solution and let grow until they come together, as the point of the test is to see how cells grow when they are together with different cell types, and to see if it is possible to control the overall concentrations of the cell types by just changing the arrangement of the original cell cluster.
Because we plan to be able to use any cell types for any of the colonies it is important to come up with a model which can explain the cell growth of all 3 cell types over a period of time.
Where:
GA = Total cell growth of A with respect to time
nA = Cell growth of A with no external forces acting
SB = Concentration of cell type B
CBA = Constant of affect (how B affects A)
SC = Concentration of Cell type C
CCA = Constant of affect (how A affects B)
The constants of affects will be determined experimentally and the concentrations can then be found by rearranging the equations. In order to obtain the constants you would need to stick cells together with known concentrations, for example to find CCA you would stick C to A and monitor how quickly A grows. CCA would then be equal to this value divided by the original growth rate of A.
This equation combines the above two and is the final form of the cell growth of A. The growth of B and C can be found by substituting out A for B or C appropriately.
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How Constants affect Growth
From this graph we can infer that changing the affect constants (by using different cell types) will change the cell growth rate of A. However there always appears to be a limit to the maximum or minimum growth rate as shown by the clear asymptotes of the graph.