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PCR ferritin (fr1)

Amplifying ferritin

development of ferritin

Amplified ferritin

<a class="postscriptum protocol" href="https://2015.igem.org/Team:Groningen/Protocols_and_Protocols/PCR">PCR</a>

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Ferritin sequence received from TecCEM HS and ordered at IDT. PCR ferritin sequence with prefix and suffix primers (figure 1) using NEB Q5 High-Fidelity 2X Master Mix.

Primer

Sequence

prefix

CAGGCAGTTGAATTCGCGGCCGCTTCTAGA

suffix

CTTGAGCTCCTGCAGCGGCCGCTACTAGTA

Primers for ferritin PCR.