Team:Austin UTexas

UT Austin iGEM 2015 Home

BREAKING IS BAD

Our iGEM team at the University of Texas at Austin developed five individual projects inspired by members’ interests and concerns in synthetic biology, with a foundation of technical skills and lab experience built during a spring semester course. The projects our team members have devised focus on a multitude of topics, from attempts at improving the stability and efficiency of existing genetic machines, to identifying bacterial factories that can have ecological function. Our projects focused on improving and expanding on the existing microbial factories in E. coli include an attempt to optimize the ΔguaB pDCAF strain of E. coli (Quandt et al., 2013) to discount nutrients provided by non-caffeine methylxanthines, and a project assessing the evolutionary stability of yellow fluorescent protein, enhanced yellow fluorescent protein, and super-folder yellow fluorescent protein.

Fluorescent protein-producing bacteria cultures grown over 10 days. One culture was grown each day by moving a small sample of the previous day's culture into new LB media.

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CAFFEINATED COLI

redesign for greater stability

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References

Quandt, Erik M., et al. "Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5." ACS synthetic biology 2.6 (2013): 301-307.

INTERLAB STUDY & BREAKING IS BAD

what does BB have to do with interlab?

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