Team:Groningen/Notebook/Restriction fr12
Blue Bio Energy
Restriction pSB1C3 (fr12)
Digestion of pSB1C3 for ligation with ferritin
Development ferritin
Digested product
Restriction
16:00, 31 August 2015 - 18:30, 10 August 2015
Digest pSB1C3 in order to ligate them. Samples were digested for 2h at 37°C.
#
Component
Amount
2
pSB1C3
10 µL
10x buffer (2.1)
2 µL
PstI
1 µL
EcoRI
1 µL
\( \mathrm{H_2O}\)
6 µL
Components for digestion.