Team:CSU Fort Collins/Results
COLORADO STATE
UNIVERSITY iGEM
Project Results
Key Results
Key Achievements- Construction and submission of multiple team parts
- Proof of the composite lac promoter:fadD:fadL part function
- Characterization of KillerRed in E. coli for use as an induced lethality switch
Key Challenges
- Reworking breakdown construct ideas after fadD experiment
- Inconclusive results about trans-zeatin production
- Proper handling and experimental design for testing KillerRed
Breakdown Results
Trans-zeatin Production Results
We ran a growth experiment to assess how much metabolic stress, if any, our construct introduced into our strain. From the results, we can conclude that the genes do not increase metabolic stress on the cell.
Figure 1: Growth comparison between a control strain and our enhanced strain.
Kill Switch Results
Scale-Up Results