Team:HUST-China/Part Collection
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Particularly,we did well in the standardization of E.coli 50S ribosomal protein (Si-tag) with the different combination of domains:1,2,3,1+2,1+3,2+3,1+2+3,1L3.
We added a signal peptide and cell wall protein respectively at N terminal and C terminal of the Si-tags which can immobilize the working protein on the cell wall to promote the protein’s function.
The results of fluorescence immunoassay showed that we succeeded in displaying the silica-tag onto the cell surface. Silica surface binding test shows each protein domain had different binding ability, so that we can choose different combinations of Si-tag domains to satisfy different requirement of binding intensity.
| Part Number | Description | Nick Name |
|---|---|---|
| BBa_K1592007 | LIP prepro + E. coli ribosomal protein L2 (1-60) + YLcwp3 Fusion | Si-tag1 |
| BBa_K1592008 | LIP prepro + E. coli ribosomal protein L2 (61-202) + YLcwp3 Fusion | Si-tag2 |
| BBa_K1592009 | LIP prepro + E. coli ribosomal protein L2 (203-273) + YLcwp3 Fusion | Si-tag3 |
| BBa_K1592010 | LIP2 prepro + E. coli ribosomal protein L2 (1-202)+ YLcwp3 Fusion | Si-tag12 |
| BBa_K1592011 | LIP prepro + E. coli ribosomal protein L2 (61-273) + YLcwp3 Fusion | Si-tag23 |
| BBa_K1592012 | LIP prepro + E. coli ribosomal protein L2 (1-60,203-273) + YLcwp3 Fusion | Si-tag13 |
| BBa_K1592013 | LIP prepro + E. coli ribosomal protein L2 (1-60,GS linker,202-273) + YLcwp3 | Si-tag1L3 |
| BBa_K1592014 | LIP prepro + E. coli ribosomal protein L2 (1-273) + YLcwp3 | Si-tag123 |