Team:CSU Fort Collins/Results

COLORADO STATE
UNIVERSITY iGEM

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Project Results

Contents

1 Key Results
2 Breakdown
3 Trans-zeatin Production
4 Kill Switch
5 Scale-Up
6 References

Key Results

Key Achievements

• Construction and submission of multiple team parts
• Proof of the composite lac promoter:fadD:fadL part function
• Characterization of KillerRed in E. coli for use as an induced lethality switch

Key Challenges

• Reworking breakdown construct ideas after fadD experiment
• Inconclusive results about trans-zeatin production
• Proper handling and experimental design for testing KillerRed

Breakdown Results

Trans-zeatin Production Results

We ran a growth experiment to assess how much metabolic stress, if any, our construct introduced into our strain. From the results, we can conclude that the genes do not increase metabolic stress on the cell.

Figure 1: Growth comparison between a control strain and our trans-zeatin strain over 24 hours.

Kill Switch Results

Scale-Up Results

References

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