Team:Liceo Eugenio Hostos/Experiments
Experiments & Protocols
Protocols
- Bacterial transformation:
- remove the biobriks that come in kits plates.
- Put 10 cl water HQ in the well and pump that blended leaving 10 ul of a red substance. Then put the substance in had ependorf.
- It should then be left with chemo-competent bacteria.
- Introducing: 100 ul of bacteria.
- Add 250 ul only to bacteria.
- 2 ul plasmido.
- 10 cold 0 °.
- 42° min 50 sec Thermo blocked.
- 2 min 0 °.
- 10 min T ° ambiente.
- LB+Antibiotico.
- Lb+CHLOR.
- LB+Bacteria.
- LB+Biobrick CHLOR.
- 5ml.
- 5 antibiotic to Biobrick.
- LB CHLOR.
- 10 ul min T ° environment.
- Place all the bacteria in a culture.
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration