Experiments & Protocols
Protocols
Bacterial transformation:
remove the biobriks that come in kits plates.
Put 10 cl water HQ in the well and pump that blended leaving 10 ul of a red substance. Then put the substance in had ependorf.
It should then be left with chemo-competent bacteria.
Introducing: 100 ul of bacteria.
Add 250 ul only to bacteria.
2 ul plasmido.
10 cold 0 °.
42° min 50 sec Thermo blocked.
2 min 0 °.
10 min T ° ambiente.
LB+Antibiotico.
Lb+CHLOR.
LB+Bacteria.
LB+Biobrick CHLOR.
5ml.
5 antibiotic to Biobrick.
LB CHLOR.
10 ul min T ° environment.
Place all the bacteria in a culture.
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project
Inspiration
