Team:Cooper Union/Experiments
Cooper Union 2015 iGEM
Experiments & Protocols
Bacteria Media w/ agar (solid media)
Materials
500mL bottle
Deionized Water
Agar
LB Broth
Chloramphenicol
Yeast Extract
Bacto Peptone
- Add 250mL of deionized water to the 500mL bottle.
- Weigh out 3.625g of agar using the weighing boat and add it to the 250mL of water.
- Weigh out 5.0g of LB Broth using the weighing boat and add it to the 250mL of water.
- Screw lid on bottle loosely.
- Place autoclave tape on the lid of the bottle.
- Place tray with approximately 1cm of water in height into the autoclave.
- Place 500mL bottle into autoclave with "liquid" selected and press "start".
- After an hour, remove bottle from autoclave. Let bottle sit for approximately 30 minutes.
- Add 370uL of chloramphenicol to the 250mL solution.
- Pour solution into petri dishes, covering the bottom of the dish.
- Place two stripes on each dish.
- Let dishes sit overnight.
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