Team:LZU-China/chz index
Abstract
As the global industrialization has been developed, heavy metal ions became assignable factors of water pollution, which may cause serious damage to human health. However, the measurements for the concentration of heavy metals are complex and expensive. In our project, we have applied several transcription factors which are sensitive to the concentration of copper ions and chromium ions to regulate the expression of protein ribB. The existence of ribB will enhance the production of riboflavin. Moreover, riboflavin, as the electron carrier, could improve the electricity producing performance of the Microbial Fuel Cells (MFCs). So in this way, we successfully correlated the concentration of heavy metal ions together with the output voltage of the MFC. What¡¯s more, we further created a convenient device¡ªMicro Holmes (a combination of MFC and electronic components), to monitor heavy metal ions of water sample any place any time.
Contents
We've done an investigation about water pollution in the past few months. Through street interview and online questionnaires, 1,000 valid replies were collected from 4 countries. Here's the conclusion: Althrough different countries' water pollution is in different degrees. People are very worried this. What's more, some people are urging a both cheap and convenient method to tell them whether the water is safe to drink. And that's the reason why we do this project.
We had constructed 14 different parts. All of them, including several ion sensors, have ribB CDS. Our experiments had confirmed that the expression degree of ribB can influence the riboflavin production. Then we had taken them into MFC and the voltage showed that our genetic engineered E.coli could response the change of Cu2+ concentration. Thus we linked the concentration of metal ion to the electricity production.
Based on the wet lab results, we constructed the ¡°Micro Holmes concentration measurement system¡± which contained a Microbial Fuel Cell, a Microprogrammed Control Unit, a voltage magnification circuit board, an Analogue-to-Digital Conversion chip, a set of input and output devices and one or several kinds of bacteria.
High Lgihts
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Modal Fullfillment
Bronze
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filter_dramaRibB
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1. RibB protein is able to increase and stabilize electrical current generated by microbial fuel cells. Expression level of ripB is correlated with MFC voltage.
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placeCopper
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Copper, Mercury, zinc, chromium sensors were used to regulate ripB gene's expression in bio-engineered strains and concentrations of heavy metals are correlated with RipB protein production.
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When grown our bio-engineered strains in the anode of MFC, voltage is correlated with heavy metal ion concentration.
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A novel device for heavy metal ion detection/measurement has been developed based on our findings. Comparing to current heavy metal measurement instruments, our device is simpler, cheaper and easier to use.
Sliver
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Gold
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