Team:EPF Lausanne/Results

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Results

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In order to build dCas9-controlled circuits, we aim to use dCas9-inducible synthetic promoters to mimic the behaviour of a transistor (Go take a look at our design (LINK) if you haven't !). To see whether such promoters could be used in wider-scale circuits, we identified and tested the following desirable properties :

  • abc
  • def

References

[1] Farzadfard, F., Perli, S. D., Lu, T. K. (2013). Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas. ACS Synth. Biol., 2 (10), pp 604–613.




EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

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