Team:UC San Diego/Notebook

TIMELINE

September 24-28

Giant Jamboree!

Giant Jamboree!

September 16

Library presentation at La Jolla Riford Library.

Read more! WEEK 14

Computational

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 14

Wet Lab

+Final preparations before the wiki freeze.

WEEK 14

Title of section 2

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum aut hic quasi placeat iure tempora laudantium ipsa ad debitis unde?

Read more about what we did! WEEK 13

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 13

Wet Lab

+ Mapped out errors in the parts that we assembled
+ Mutagenesis to fix recurring errors in C sequence

WEEK 13

September 4

High school meet up.

Read more! WEEK 12

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 12

Wet Lab

+ Attempted Gibson Assembly with CDE with AB
+ Designed sequence primers
+ Miniprepped full size clones

WEEK 12

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 11

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 11

Wet Lab

+ Attempted full gibson assembly of AB fragment
+ Miniprepped CDE fragments

WEEK 11

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 10

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 10

Wet Lab

+ Selected error free clones for CDE fragments from sequencing data
+ Designed primers for PacBio Sequencing
+ Transformed error free clones of CDE

WEEK 10

week 9

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 9

week 9

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 9

Wet Lab

+ Assembled using Gibson Assembly and sequenced CDE fragments
+ PCR and transformed fragments

WEEK 9

August 6

Southern California iGEM Meetup

Read more! Week 8

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 8

Wet Lab

+ Received our DNA fragments from SGI.
+ Attempted to PCR and transform our fragments.

WEEK 8

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 7

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 7

Wet Lab

+ Assembled Interlab Devices. Resuspended and measured them successfully.
+ Prepared YPD plates.

WEEK 7

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 6

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 6

Wet Lab

+ Continued interlab study with miniprep, restriction digests, ligation, gel electrophoresis and gel purification.

WEEK 6

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 5

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 5

Wet Lab

+ Started interlab study with transformation.

WEEK 5

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 4

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 4

Wet Lab

+ Sent our genes to SGI and ordered lab supplies. Almost ready to go!

WEEK 4

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 3

Section 13

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Iusto, optio, dolorum provident rerum.

WEEK 3

Wet Lab

+ Added frp gene sequence from Vibrio Harveyi to our plasmid to stabilize the production of luciferase.
+ Optimized our plasmid sequences.

WEEK 3

Website

More website ideas.

WEEK 2

Computational

Read papers on drive
Read about metabolic control analysis
Compiled notes on bioluminescent system
Literature research:understanding bioluminescent mechanism
> aldehyde synthesis
> FMNH2 production
> light production
Familiarized with COBRA module

Primer to Genome-Scale Modeled and COBRA Tutorial (Jahir)

Continued literature search for key mechanisms regarding our auto-induced bioluminescent reaction in a yeast model
>auto-induced therefore no coupling between cells (removed LuxI and LuxR genes)

WEEK 2

Wet Lab

+ Created a preliminary design for the plasmids using ApE.
+ Improved them over the week by adding tags, removed illegal restriction sites, and changing repetitive sequences.

WEEK 2

Website

Discussed and designed website prototypes.

WEEK 1

Computational

Developed Primer to MATLAB Programming
> lecture material from MIT course and UCSD course
> reading material from UCSD course
> study and practice material MIT course and UCSD course

WEEK 1

Wet Lab

+ Found genes coding for fatty acid reductase complex that have been validated in an in vitro synthesis paper.
+ Planned how to assemble our plasmids and determined nucleotide sequences for lux A-E of Photobacterium Phosphoreum.
+ Compared the amino acid sequences to those of other organisms on BLAST to check for significant discrepancies in our sequence.

WEEK 1