Preparation of Stock Solutions
1. gBlocks
The gBlocks ordered from IDT arrived in the form of vials of 200ng solid DNA powder.
(refer to BioBricks page for information on DNA sequences)
The gBlocks were made into 10ng/µl stock solutions in Milli-Q water for storage:
mass / ng |
conc / ngµl-1 |
final volume / µl |
200 |
10 |
20 |
2. Primers
The forward and reverse primers ordered from IDT came in 32.4nmol and 34.3nmol of solid respectively.
(Sequences: Forward - CTTTTTTGCCGGACTGC; Reverse - ATGATTTCTGGAATTCGC)
The primers were made into 100µM stock solutions in Milli-Q water for storage:
amt / 10-9 mol |
conc / 10-6 M |
final volume / 10-6 L |
32.4 |
100 |
324 |
34.3 |
100 |
343 |
Preparation of Reaction Solutions
1. gBlocks
2µl of each stock solution were diluted in Milli-Q water to achieve final solution volumes of 20µl to make 1ng/µl-1 reaction solutions.
2. Primers
2µl of each stock solution were diluted in Milli-Q water to achieve final solution volumes of 20µl to make 10µM reaction solutions. (These solutions are labelled as “Prefix primer” and “suffix primer” in eppendorf tubes in the fridge)
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