Team:CHINA CD UESTC/Journal
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JOURNAL
Hi! Here recorded the “significant” stages of our team which we experienced throughout the whole summer. In the initial establishment of our team, we organized various social activities and uneasy demonstrated the project at the first time, which all presents to you here.
Journal
The establishment of our team!
Came up with gas detector that examine institution and several diseases of our body.
Put forward a kind of novel plant paint which can paint on the plant surface to absorb PM2.5.
Catch the idea of yeast probiotic yogurt which helps people who allergic to alcohol to rapid degradation of ethanol.
We had a brain storming: tracer showing the concentration of biomolecules, plants can emit colored light, new type of bacterial cell, and wastewater treatment by magnetotactic bacterium, etc.
We established the iGEM club in our campus.
Determined the project as "magnetotactic E.coli generates electronics."
Ascertained the origin of mamW gene in MSR-1 genome.
Ascertained the origin of laccase gene - derived from Escherichia coli.
Designed the primers of mamW, RFP and laccase.
Purchased the laboratory consumables and prepared for the first human practice activity.
Communicated with professor Hu from Hong Kong University Medicine School.
Determined three vectors for co-transferred which are pACYCDuet-1, pET28a and pCDFDuet-1.
Amplified the mamGFDC and mms6 with PCR from MSR-1.
Linearization of the pACYCDuet-1 and amplification of the mamW, RFP and laccase.
Presentation and science lesson at the experimental Kindergarden Affiliated to UESTC.
Presentation and Interaction at the Primary School Affiliated to UESTC
Four teaching teams’ from iGEM club departure towards schools in remote area.
Our summer vocation began, in order to have fun before the summer iGEM training, we go out for an outing!
Screened positive clones of mamW + RFP + laccase and RFP + laccase.
The start of the Summer Training Camp.
The establishment of the iGEM Southwest Union of China, also the first meet up.
We transferred mamW, RFP and laccase gene into Escherichia coli BL21 and induced its expression.
Connected mamAB with the vector pET28a and complete the construction of vector piGEM-pET28a-mamAB.
Amplified the mamXY with PCR from MSR-1. Completed the construction of vector piGEM-AB.
Communication with technical expert at Anno Medical Devices Co.Ltd.
Detection of laccase activity using by the ABTS. Communication with Manager Zhiyong Xu at Jin Douyun Space Company.
Finished the project video.
Connected mamXY with the vector pCDFDuet-1 and connected mamGFDC + mms6 with the vector pCDFDuet-1-mamXY.
We visited Chengdu Biology Maker Space and got their technical support.
We were excited to obtain the exact Parts including the mamW gene and mutanted it.
We completed our enzymatic biofuel cell! It worked as we expected!
The first time we presented our whole project presentation in front of all instructors and professors of our school.
It is an exciting day that we sent our Parts to MIT, wish the little box bon voyage. Happy to complete the construction of cell device and start to test it.
Team members gathered together to modify the wiki for the finalist version under the guidance of our instructors.
We completed our wiki and poster, which means we completed the basic standards of medals. Next is the comprehensive preparation of presentation.
Set out for 2015 iGEM Giant Jamboree in MIT. We are coming, we are CHINA_CD_UESTC!