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HRP-mimicking DNAzyme

The versatile HRP-mimicking DNAzyme forms a G-quadruplex structure in which hemin can be incorporated.Travascio1998 This enables it to catalyze the fission of hydrogen peroxide to water and a reactive oxygen species (ROS). Thus it can be used to catalyze chemiluminescence and a series of colorimetric reactions, known from the horseraddish peroxidase from Amoracia rusticana (Fig.3). As we show in our AptaBody project the HRP-mimicking DNAzyme can be used to generate a signal on a western blot. Here we directly blot the DNA onto the membrane and recover its activity after denaturing polyacrylamide gel electrophoresis (PAGE). For this after the transfer the catalytically important hemin is added to the DNAzyme which results into catalytic activity on the membrane.