Team:Lethbridge HS/Results

Results

What results did we get in the lab?

• Description

• What is nuclease? What is dextranase?

• What we are doing differently

• Extracellular Polymeric Substance Matrix

Results

Oxalic acid results:

Oxalic Acid Concentration (mg/mL)	OD528
0	1.7104
0.01	1.817 (only one trial)
0.5	1.647 (only one trial)
0.1	1.145125
0.15	1.6255 (only two trials)
0.2	0.7021428571
0.25	1.5195 (only two trials)
0.3	0.588375
0.35	1.696 (only one trial)
0.4	0.7051
0.45	1.737 (only one trial)
0.5	0.6271428571
0.55	1.666 (only one trial)
0.6	0.6502222222
0.65	1.674 (only one trial)
0.7	0.6052857143
0.75	1.597 (only one trial)
0.8	0.7459
0.85	1.533 (only one trial)
0.9	0.504
1.0	0.6849
1.4	0.789 (only two trials)
1.5	0.983 (only one trial)
1.6	0.8195 (only two trial)
1.8	0.047 (only one trials)
1.6	0.037 (only two trials)

Bee trials:

Treatment	Before	After
None	Somewhat lethargic. Drank when prompted.	Appeared tired, but otherwise unaffected.
0.00%	Most lethargic, did not eat when offered	Wet and slow moving.
0.05%	Very vigorous; ran around inside the tube. Fed.	Very energetic. Unaffected by treatment.
0.2%	Very vigorous; ran around inside the tube. Fed.	Slightly less energetic than the 0.05% bee.

The bees will be able to survive all of the oxalic acid that the E.coli can produce.

Mite trials:

	Control	Oxalic Acid	RFP
Parafilm Pouch	Mites dead	Mites dead	Mites dead
Larvae 1	Mites dead	Mites dead	Mites dead
Larvae 2	Mites dead	Mites dead	Mites dead

The mites did not survive any of the trials.

Antibiotics testing:

Test (Antibiotics)	Result	After
AMP 1	Positive
AMP 2	Positive	Wet and slow moving.
CAM 1	Positive
CAM 2	Positive
TET 1	Positive
TET 2	Positive
Control (water)	Positive

The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.