Team:York/Part Collection

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Part Collection

Source Organism	Gene	Part Name	Function	Characterised	Sequenced	Submitted
Expression vector “pAdapt” in pSB1C3	lacZalpha	BBa_K1807000	Used as cloning vector	✔	✔	✔
Escherichia coli	EcPPX	BBa_K1807001	Phosphate Kinase		✔	✔
Escherichia coli	EcPPK	BBa_K1807002	Phosphate Kinase		✔	✔
Escherichia coli	EcPstSCAB		Phosphate specific transporter		✔
Kingella oralis	KoPPK	BBa_K1807006	PolyPhosphate Kinase		✔	✔
Sinorhizobium meliloti	SmPstSCAB		Phosphate specific transporter	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK BA-91	BBa_K1807003	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK SK-12	BBa_K1807004	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPPK UW-1	BBa_K1807005	PolyPhosphate kinase	✔	✔	✔
Candidatus Accumulibacter phosphatis	ApPstSCAB	BBa_K1807007	Phosphate specific transporter		✔	✔

Below is a plate imgage of blue-white screening using X-Gal/IPTG, proof of transformation with our SmPst genes.