humanpractice

Collaboration

Since firstly taking part in iGEM, NJU-China has the tradition of communicating with other teams in China. We do not only exchange our ideas and experiences with others, but also spread the knowledge of synthetic biology as well as iGEM Competition in China. This year we kept the tradition and took part in several activities. These activities strengthened our understanding of synthetic biology and deepened the friendship with other teams. We benefit each other in a mutual way.

Collaboration with SYSU-Software

We cooperated with the Software team from Sun Yat-sen University to develop the conditioned place preference (CPP) mice image recognition software, and utilize the software into our CPP test to record the reflection of mice. The software is able to present the statistic results in the form of heat maps, reflecting the movement of mice directly and vividly. We thank our collaborator for receiving us with attention and for such strong contribution to our work.

In this experiment, we have seven mice. Each mouse is placed in a pair of interconnected cages, including a bright one and a dark one. Our experiment requires us to record the mice movement in order to accomplish the following goals:
1.To tell which cage the mice prefer to stay, bright one or dark one
2.Record the mice coordinate precisely

It’s hard for us to finish such job in tradition way. So we decided to seek some software to assist us. However, the software available to us can not meet our demands. It is really tricky. Fortunately, in iGEM China Community, we contact SYSU-Software, a well-known software team and ask them to produce a tool for us. With their tool, we record and analyze the video. Finally we obtain the following results:
1.The data of the mice coordinate, 30 times per second
2.Based on the data, the tool can generate a Heat Maps, from which, we can know which cage the mice prefer to stay
Once again, we would like to thank SYSU-Software.

Collaboration with NUDT_China

1.NUDT_China helped us to perform nanoparticle tracking analysis (NTA) to have a more precise determination of the quantity and size of secreted exosomes. They did a supereminent experiment for us and we collected the perfect data we want.
2.We also helped NUDT_China to construct a TAL effector. The recognition sequence of TALE1 (provided by NUDT_CHINA): 5’-GGAGGCACCGGTGG -3’ (14bp)
The corresponding RVD: NN NN NI NN NN HD NI HD HD NN NN NG NN NN

Figure 1 Sequence of TALE1 cDNA and deduced amino acids.

The construction of this implemented with Golden Gate TALEN and TAL Effector Kit 2.0 by Addgene.
We then reedited the vector pTAL1 provided by the kit to add EcoRI, SpeI and PstI cutting cite to make the TALE we built using this vector can meet the RCF10 standard.

Conferences with NJAU_China

We held several project intercommunion meetings with Nanjing Agricultural University and exchanged our ideas and experiences. During the meetings, we provided some suggestions on their project. We also offered many plasmids to support their experiments efficiently.

Mentoring FAFU-CHINA

We helped the iGEM team from Fujian Agriculture and Forestry University in their topic revision and provided them with some suggestions. We also provided a vigorous support for their follow-on experiments.

Offering Parts