Team:UiOslo Norway/Experiments/Restriction Digest

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Restriction Digest:

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5 μl Cutsmart
x μl DNA (1 μg total concentration)
1 μl Restriction Enzyme I
1 μl Restriction Enzyme II
up to 50 μl MilliQ Water

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Total Reaction of 50 μl

Incubate for 1 hour at 37 °C

For restriction digests including NdeI, at first only 1 μl of NdeI was added and the digest incubated at 37 °C for 1 hour, afterwards NdeI was heat inactivated at 65 °C for 20 minutes and then the second restriction enzyme was added and the digest was again incubated for 1 hour.

iGEM UiOslo 2015 is sponsored by:

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