Team:Westminster/Results

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Project Results

Our plan from the beginning was to express the MtrCAB operon found in Shewanella oneidensis MR-1 in Escherichia coli along with research the potential benefits the proteins CymA and OmcA would have on a microbial fuel cell. From reviewing the literature we quickly discovered that cloning these genes into E.coli would be a challenge as they can be potentially toxic to the cell. Due to this we decided to try to express each gene individually with His-10 tags to show that they can be expressed in E.coli.

Westminster team took advantage on the generous offer from IDT and decided to get the five genes that were the focus of this project (MtrA, MtrB, MtrC, CymA, OmcA) synthesised as gBlocks. As we were planning to use the novel RDP cloning technique designed by Synbiota we had to order 20 primers to convert the gBlocks into BioBrick and RDP formats, these were designed in SnapGene to have GC clamp and annealing temperature of 60-70°C

Click here to see details of our lab results