Team:KU Leuven/Research/Basic Part

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Research

Interlab

Modeling

Outreach

Future

Team

Notebook

main

Research

Interlab

Modeling

Outreach

Future

Team

Notebook

Basics Parts

On this page you can find all our basic parts needed for the system to work. To learn more about them, click the titles below!

Name	Construct	Length	Description
BBa_K1709002	CheZ-GFP	1401 bp	text
BBa_K1709000	LuxI-His	609 bp	text
BBa_K1709004	LuxR-E	835 bp	text
BBa_K1709005	Ag43-YFP	3843 bp	text

CheZ-GFP

LuxI-His

Theory

Motility Test Assay

Protocol

1. Prepare selective media (LB with 0.25% agar (2,5 g/L) in Petri dishes (85 mm dia).

2. Apply 1.5 µL of the diluted cell suspensions from mid-log-phase cultures (~2×105 cells/µL (OD=0.5)) to the center of the plates, and let dry in air for 15 min.

3. Incubate at 37 °C for 10 h.

Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

The results of our experiments will appear here.

Back

Go back to the Research page.

Idea

A detailed description about the interaction between our two cells and the genetic circuit can be found here.

Methods

Here you can find the performed steps to create two different cell types and detailed quantification methods to determine interesting parameters.

Composite Parts

Our basic parts were combined with each other and with existing iGEM promotors, RBS and terminators.

Results

Click here to discover our results

Back

Go back to the Research page.